Synaptophysin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91307-50, BS91307-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08247(human) Q62277(mouse) P07825(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Synaptophysin polyclonal antibody detects endogenous levels of Synaptophysin protein.

Bioworld Molecular Weight: 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.
Positive control:
Lane 1: Human brain
Lane 2: PC-12, ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SYP, Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Synaptophysin, Syp, SYPH, SYPH, SypI,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified