Ubiquitin (K63-linkage Specific) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91401-50, BS91401-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0CG47(Human) P0CG48(Human) P62979(Human) P62987(Human) P0CG49(Mouse) P62991(Mouse) P0CG51(Rat) P62989(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S Proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lys 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκB-α and p27.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Ubiquitin (K63-linkage Specific) polyclonal antibody detects endogenous levels of Ubiquitin (K63-linkage Specific) protein.

Bioworld Molecular Weight: 60-100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution., ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: UBB, UBC, RPS27A, UBA52, Epididymis secretory protein Li 50, FLJ25987, HEL S 50, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified