VCP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91425-50, BS91425-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55072

Host: Rabbit

Reactivity: Human, Mouse, zebrafish

Applications: WB, ICC IHC

All Applications: WB:1:2,000
ICC:1:200
IHC:1:200

Background: Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VCP polyclonal antibody detects endogenous levels of VCP protein.

Bioworld Molecular Weight: 89 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.
Positive control:
Lane 1: L929
Lane 2: F9
Lane 3: A549
Lane 4: MCF-7
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: N2A
Lane 8: Jurkat
Lane 9: HepG2
Lane 10: A431
Lane 11: Mouse heart
Lane 12: Mouse brain
Lane 13: Mouse liver
Lane 14: Mouse kidney, ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TER ATPase, VCP, 15S Mg(2+) ATPase p97 subunit, 15S Mg(2+)-ATPase p97 subunit, ALS14, ATPase p97, CDC48, IBMPFD, MGC131997, MGC148092, MGC8560, p97, TER ATPase, TERA, TERA, Transitional endoplasmic reticulum ATPase, Valosin containing protein, Valosin-containing protein, VCP, Yeast Cdc48p homolog,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified