TDP43 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91326-50, BS91326-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13148(Human) Q921F2(Mouse) Unigene:2633(Rat) EntrezGene:325052(Zebrafish)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, IP, ICC/IF IHC FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TDP43 polyclonal antibody detects endogenous levels of TDP43 protein.

Bioworld Molecular Weight: 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TDP43 on different cells lysates using anti-TDP43 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: K563, ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: RNA polymerase II subunit B1, POLR2A, ALS10, OTTHUMP00000002171, OTTHUMP00000002172, OTTHUMP00000002173, TADBP, TAR DNA binding protein 43, TAR DNA binding protein, TAR DNA-binding protein 43, TARDBP, TDP 43, TDP-43, TDP43,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified