TH Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91342-50, BS91342-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07101(Human) P24529(Mouse) P04177(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:50,000-1:100,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TH polyclonal antibody detects endogenous levels of TH protein.

Bioworld Molecular Weight: 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Tyrosine Hydroxylase on different lysates using anti-Tyrosine Hydroxylase antibody at 1/50,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Mouse brain, ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TH, Dystonia 14, DYT14, DYT5b, EC 1.14.16.2, OTTHUMP00000011225, OTTHUMP00000011226, ple, Protein Pale, TH, The, TY3H, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, Tyrosine hydroxylase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified