EYA1/4 Polyclonal Antibody

Catalogue Numbers: BS60698-50, BS60698-100

Sizes: 50µl, 100µl

Alternative Name: Eyes absent homolog 1; EYA1; Eyes absent homolog 4; EYA4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99502/O95677

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: A gene on chromosome 8q13.3 encodes EYA1 (eyes absent), a protein with 16 exons. EYA1 is one of four members of the eyes absent family. A 271 amino acid domain at the carboxyl terminal is highly conserved amongst the members of the eyes absent family, while the PST (proline-serine-threonin)-rich amino terminal is highly divergent. EYA is expressed in flexor tendons and the developing central nervous system, kidney, eye and ear. EYA1 acts a transcriptional activator in connective tissue patterning through its PST domain, which functions as a transactivation domain. EYA1 plays a critical role in the development of the inner ear and kidney. EYA is involved in early inductive signaling, acting upstream of GDNF. EYA1 has been implicated in the autosomal dominant disorders branchio-oto-renal (BOR) syndrome and branhio-oto (BO) syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EYA1/4 polyclonal antibody detects endogenous levels of EYA1/4 protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EYA1/4 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human EYA1/4

Conjugate: Unconjugated

Modification: Unmodified