The ubiquitin specific protease 3 USP3 is a deubiquitinating enzyme for uH2A and uH2B. USP3 dynamically associates with chromatin and deubiquitinates H2A/H2B in vivo. The ZnF-UBP domain of USP3 mediates uH2AUSP3 interaction. Functional ablation of USP3 by RNAi leads to delay of S phase progression and to accumulation of DNA breaks, with ensuing activation of DNA damage checkpoint pathways. In response to ionizing radiation, (1) uH2A redistributes and colocalizes in g-H2AX DNA repair foci and (2) USP3 is required for full deubiquitination of ubiquitin-conjugates/uH2A and g-H2AX dephosphorylation. USP3 is a novel regulator of H2A and H2B ubiquitination, highlight its role in preventing replication stress, and suggest its involvement in the response to DNA double-strand breaks1. USP3 has been characterized as a functional DUB in vitro, and it is the human DUB most homologous to S. cerevisiae Ubp8, which regulates H2B deubiquitination2-4.

Product information

Molecular Weight: 937.07

Formula: C38H68N10O15S

Chemical Name: 2-{2-[2-(2-{2-[2-(2-{2-[2-(2-amino-3-hydroxypropanamido)-3-hydroxybutanamido]-3-hydroxybutanamido}propanamido)-3-methylpentanamido]-3-sulfanylpropanamido}propanamido)-3-hydroxybutanamido]acetamido}-4-methylpentanoic acid

Smiles: CC(C)CC(NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(CS)NC(=O)C(NC(=O)C(C)NC(=O)C(NC(=O)C(NC(=O)C(N)CO)C(C)O)C(C)O)C(C)CC)C(C)O)C(O)=O

InChiKey: FDKQXVATCPHLKJ-UHFFFAOYSA-N

InChi: InChI=1S/C38H68N10O15S/c1-10-16(4)26(45-30(54)18(6)42-36(60)28(20(8)51)48-37(61)29(21(9)52)47-32(56)22(39)13-49)35(59)44-24(14-64)33(57)41-17(5)31(55)46-27(19(7)50)34(58)40-12-25(53)43-23(38(62)63)11-15(2)3/h15-24,26-29,49-52,64H,10-14,39H2,1-9H3,(H,40,58)(H,41,57)(H,42,60)(H,43,53)(H,44,59)(H,45,54)(H,46,55)(H,47,56)(H,48,61)(H,62,63)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.