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home > ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml > ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml

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ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 mlROTILux SYBRGreen qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml, Density (D) ~1 g cm, Boiling point (bp) 100 C, Storage temp. 20 C ROTILux SYBRGreen qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real time PCR. The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one

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ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml, Density (D) ~1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C

ROTI^®Lux SYBR^®Green qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real-time PCR.The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (>10x).

Properties:

High temperature stability
Excellent sensitivity (<0.1 pg cDNA)
Ultra-fast qPCR in <20 min
Suitable for melting curve analysis (HRM)
For all common qPCR cyclers - optionally with or without ROX

Applications:

Gene expression analyses
Melting curve analyses (HRM)
Genotyping
Concentration determination of NGS libraries
Determination of copy number variants
Telomere length measurement
Pathogen detection
Quality control

Master mix with low concentration (100 nM) of the reference dye ROX (6-Carboxy-X-rhodamine)

Suitable for:

Quantstudio™ Cycler ( Thermofisher)
qTower (Analytik Jena)
Mx3000P^® qPCR Systeme (Agilent)
Rotor-Gene™ (Qiagen)

Stability by multiple freeze and thaw cycles: The stability of the ROTI^®Lux qPCR master mixes is guaranteed even after multiple freeze and thaw cycles (≥10).

Sensitivity and dynamic range: The ROTI^®Lux SYBR^®Green qPCR Master Mixes deliver consistent and reliable results, even at low input amounts such as 0.1 pg cDNA, or 1 template per reaction.

Stability at room temperature: The ROTI^®Lux qPCR Mastermixes can be stored at room temperature (≤30 °C) for at least 14 days.

Not a medical device / Not an IVD product

Technical Information

Application	Gene expression analyses, SNP analyses, telomere length measurement
Basic material	DNA (cDNA / gDNA)
Polymerase	HotStart DNA Polymerase
Instructions for use, addition	The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl₂and the reaction buffer with the SYBR^®Green dye. Primer and template must be added separately.1 ml Master Mix is sufficient for one 96-well plate.
Version	low ROX

Technical Documentation (PDF)

ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml

Name: ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml
Brand: lifisolutions.eu
SKU: 98540314198
Price: 80.65 USD
Availability: InStock
Rating: 4.747 (300 reviews)