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Recombinant Sortase A Enzyme | Protein Labeling & LPXTG Ligation | Amid Biosciences
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Recombinant Sortase A Enzyme | Protein Labeling & LPXTG Ligation | Amid Biosciences
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Recombinant Sortase A Enzyme | Protein Labeling & LPXTG Ligation | Amid BiosciencesStaphylococcus aureus Sortase A (srtA) belongs to a class of transpeptidases that utilize an active site cysteine thiol to modify proteins by recognizing and cleaving a carboxy terminal sorting signal, LPXTG (where X is any amino acid), between the threonine and glycine residues. The terminal glycine is then replaced with any poly Glycine (Gly)n label (where n= 3 or more glycine residues) to attach HPR, biotin, fluorophores and other labels. The
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Staphylococcus aureus Sortase A (srtA) belongs to a class of transpeptidases that utilize an active site cysteine thiol to modify proteins by recognizing and cleaving a carboxy-terminal sorting signal, LPXTG (where X is any amino acid), between the threonine and glycine residues. The terminal glycine is then replaced with any poly-Glycine (Gly)n label (where n= 3 or more glycine residues) to attach HPR, biotin, fluorophores and other labels. The enzyme is calcium dependent.Ê
Recombinant Sortase A Enzyme | Protein Labeling & LPXTG Ligation | Amid Biosciences
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US$ 250.00
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