SARS-CoV-2 ORF3A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80249-50, BS80249-100

Swiss-Prot: P0DTC3

Host: Rabbit

Reactivity: SARS-CoV-2

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporin localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosomes formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of 293T cells, using SARS-CoV-2 ORF3A antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Bioworld Molecular Weight: 35KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide of coronavirus ORF3A(YP_009724391.1).

Conjugate: Unconjugated

Modification: Unmodified